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Assessment of inter- and intra-cultivar variations in olive using SSR markers Scientia Agricola
Ipek,Ahmet; Barut,Erdogan; Gulen,Hatice; Ipek,Meryem.
Olive (Olea europaea L.) production in the world has been made by using many cultivars, and the genetic uniformity of commercial cultivars is important for standard olive oil and table olive production. The genetic variation among and within commonly cultivated olive cultivars in Turkey was analyzed using SSR markers. A total of 135 leaf samples were collected from 11 commonly cultivated olive cultivars from 11 provinces in four geographical regions of Turkey. Seven SSR primer pairs generated 46 SSR markers, and the number of SSR markers per primer pair ranged from 4 (UDO-14) to 9 (GAPU-89) with an average of 6.57. This high level of SSR polymorphism suggests that olive production in Turkey has been made using genetically diverse olive cultivars and this...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Olea europaea L.; Microsatellite; Intra-varietal variation; Synonyms; Simple sequence repeats.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162012000500007
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Development and validation of new SSR markers from expressed regions in the garlic genome Scientia Agricola
Ipek,Meryem; Sahin,Nihan; Ipek,Ahmet; Cansev,Asuman; Simon,Philipp W..
Only a limited number of simple sequence repeat (SSR) markers is available for the genome of garlic (Allium sativum L.) despite the fact that SSR markers have become one of the most preferred DNA marker systems. To develop new SSR markers for the garlic genome, garlic expressed sequence tags (ESTs) at the publicly available GarlicEST database were screened for SSR motifs and a total of 132 SSR motifs were identified. Primer pairs were designed for 50 SSR motifs and 24 of these primer pairs were selected as SSR markers based on their consistent amplification patterns and polymorphisms. In addition, two SSR markers were developed from the sequences of garlic cDNA-AFLP fragments. The use of 26 EST-SSR markers for the assessment of genetic relationship was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Expressed sequence tags (EST); Simple sequence repeat markers; Genetic relationship.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162015000100041
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